Pyramidal cell morphology and cell death in the hippocampus of adult mice with experimentally induced hydrocephalus

Background: Hippocampus is a neural structure in the temporal lobe that plays a crucial role in learning and memory. Cognitive impairment with learning disabilities is a common feature in hydrocephalus and is more prominent in adult-onset hydrocephalus. The aim of this study is to describe the morphological alterations in the pyramidal cells of the hippocampus of adult hydrocephalic mice. Method: Hydrocephalus was induced in adult albino mice by intra-cisternal injection of kaolin suspension (250 mg/ml in sterile water). They were sacrificed 7, 14 and 21 days post-induction. Morphological analysis was carried out on hematoxylin and eosin stained coronal sections of the hippocampus: the pyramidal neurons (normal and pyknotic) in the CA1 and CA3 subregions were counted and the pyknotic index (PI) was calculated. The somatic and dendritic features of Golgi stained pyramidal neurons were examined by light microscopy in both hydrocephalic and control mice. Result: The PI was significantly greater in the CA1 region of the hippocampus in the hydrocephalic groups compared to the age matched controls. The dendritic processes of pyramidal neurons in the CA1 region were fewer with shorter terminal branches in the hydrocephalic mice than in controls; this was pronounced at 7 days post-induction. In the CA3 region, there was no difference in dendritic arborization between hydrocephalic and control mice. Conclusion: Acute adult-onset hydrocephalus was associated with increased pyknosis and reduced dendritic arborization in hippocampal pyramidal cells in the CA1 but not CA3 region

Aetiological Patterns and Management Outcome of Paediatric Head Trauma: One-Year Prospective Study

Trauma is themost common cause of paediatric deaths. In75of paediatric trauma deaths; head injury is responsible; and most are from falls. Recent reports from Nigeria; however; appear to indicate a predominance of road traffic accidents; instead of falls. To evaluate the aetiology of paediatric head trauma; management protocols and outcome from our Centre; in order to acquire a baseline data base and recommendmeasures to reduce childhood trauma. Aprospective study of all paediatric head trauma cases presenting toNnamdiAzikiwe University Teaching Hospital; Nnewi; for 12months from April 21; 2006 to April 20; 2007; was done and collated data subsequently analyzed. The paediatric age group was taken as =15years; and grading of head injurywaswith theGlasgowComa Scale (3 15) and themodified scale for non-verbal children;while outcome wasmeasuredwith the Glasgow Outcome Scale (1 5). Out of 334 patients treated within the period of study; 210 were head trauma cases. Of these; 52 were paediatric head trauma; representing 24.8of all head trauma cases; and 19.2(10 of 52) of them were aged 0- 2years. About 62(32 of 52) were males. Falls and RTA were each responsible in 25(48.1) cases. Mild head injury occurred in 31(59.6); and 49(94.2) patients were evaluated by plain radiography. Treatment was conservative in 39(75) cases;with satisfactory outcome in 36(69.2); and amortality rate of 15.4. Road traffic injury; mostly from motorcycles; has become the major cause of morbidity and mortality amongst the paediatric age group; especially the male gender; and outcome from management is mostly satisfactory